Rhizobox Gas Flux & Labelling Chambers. Measuring CO2, O2, H2 or NH4 gas exchange in rhizoboxes is important to determine plant vigor, photosynthetic performance and ecosystem C and GHG fluxes, Nitrogen fixation. We thus build respiration chambers which can be tightly placed on top of rhizoboxes, allowing for convenient measurements of gas fluxes by attaching instruments of choice, e.g. Qubit gas analyser (IRGA) or Picarro for CO2 (isotopic) measurements. Isotopes in general are versatile research tools. The VSI top-up chambers for any type of root boxes (Rhizoboxes, RhizonBoxes, mobile Rhizotrons) can thus be used as pulse isotope labelling chambers to study a range of plant and soil functions such as exudation, water use efficiency, N fixation or transfer of labelled C assimilates to roots and their symbionts.
Sleeves (black, in Fig. above) fit tightly over the boxes, the plexiglass sealing the chambers against the PMMA edge. Additional gaskets and clamps to the (rhizobox) spacers can be fitted to connect the chambers (optional). Two (or custom numbers of) gas connectors (user defined) can be used as inlets and outlets for gas flow, either for labelling, for connecting a gas analyser for open or closed chamber approach, or for chamber air circulation with external pumps. For large top pieces, we foresee an additional fan inside the chamber to ensure enhanced air mixing. Environmental parameters within chambers, particular temperature, humidity etc. can be monitored or controlled, as recommended for "regular" plant respiration chambers. We thus foresee the required openings for inserting sensor cables and/or places to connect sensor at the inside. The chambers are fully compatible with our Gas Analysers, Gas Multiplex Systems and Gas Control Systems. We build custom rhizobox and rhizotron respiration and labelling chambers - get in contact and/or select via the Rhizobox Configuration Form (pdf).