VSI IR-502 oviposition brushing machine. In insect rearing facilities, e.g. mosquito laboratories, females are often allowed to oviposit (i.e. lay eggs) in rearing cages on moist (sterilised) paper strips such as paper towels, seed germination paper or crepe paper (see below for details on oviposition paper properties). Typically, after the paper has dried, the eggs are collected by laboratory staff - using fine brushes to manually brush the eggs from the 'egg papers' into containers for maturation. However, this process is very time consuming and therefore not suitable for high-throughput mass rearing and research facilities. Together with entomologists working in mass rearing facilities (e.g. IAEA), Vienna Scientific developed and refined the VSI IR-502 brushing machine to automatically, carefully and efficiently remove and collect adherent eggs from oviposition paper strips.
Oviposition harvesting with the IR-502 involves the gradual introduction of oviposition paper strips between two counter-rotating barrels fitted with brushes. The brushing speed can be set variable. Ovipositions are subsequently collected from a removable storage tray at the bottom. The IR-502 is a straight-forward, user-friendly machine that significantly reduces the time spent collecting insect eggs in insect research and mass rearing environments. The design allows easy cleaning of all parts after removing the housing. Once the eggs are collected, they can be stored in a clean container or rearing trays with appropriate conditions, such as temperature and humidity, until they hatch.
Images for illustration purposes only, design subject to change without notice
Oviposition strips are designated strips of paper used to collect insect eggs. These strips are usually made of filter paper or other porous materials that allow female insects to lay their eggs on them. The strips are placed in the insect's habitat or breeding area in insect rearing cages and female insects are attracted to lay their eggs on the paper rather than on other surfaces. While largely depending on species-specific preferences, some general benefitial characteristics of oviposition paper strips for insect egg collection include: