Ecosystem, Plant and Soil Respiration & Isotope Labelling Chambers. One approach to estimating ecosystem or plant respiration / gas exchange is to use airtight chambers, either (i) enclosing the whole ecosystem (e.g. a mesocosm, smaller plant communities such as grasslands) and directly providing the net ecosystem CO2 exchange (NEE), or (ii) chambers enclosing only a part of the ecosystem (e.g. plant, leaves, soil (soil respiration) etc.) and thus requiring some additional up-scaling. Isotope labelling also requires airtight plant or soil labelling chambers.
Gas Flux Chamber Systems, customised plant and ecosystem respiratory chambers for field, chamber and laboratory use, constructed of transparent polycarbonate or PMMA in various sizes and geometries. If required, a modular design allows the chamber size to be increased as the plant grows. In specific, in closed chamber settings the volume can be varied by modules to make sure that the rate of increase of a gas (e.g. CO2) is small enough to avoid problems related to gas (CO2) build-up during the measurement of the flux while being well above the limits of accuracy of the CO2 gas analyser, O2 sensor, or methane analyser. Gaskets around each collar (and the hole in the baseplate, when a mesocosm is used) ensure adequate gas tightness. For field use, a metal frame can be installed along the chambers sites to ensure gas tightness to the soil. Chamber air can be homogenized by one or more adjustable (direction and speed) fans; fan size, speed and angle can be adjusted to avoid interfering with plants or measurements. A range of pipe fittings/connection plates are available for common commercial IRGA systems. A gas control system can be added, or appropriate positions for entering cables or fixing your own sensors can be foreseen.
Various different plant and soil chamber types are custom build:
As all plant and soil chambers and related gas analyser / control systems are custom-built, please include a description or sketch of your desired design, and we will be happy to arrange a meeting to discuss the details.
Overall, the construction of a gas flux / respiration chamber requires careful consideration of the materials used and environmental factors relevant in the study. Consultation with Vienna Scientific in cooperation with Qubit Systems can be helpful to ensure that the chamber design meets your specific research objectives.
Ecosystem Respiration Chambers
and Gas Flux Measurements
Custom-made Isotope Labelling Chambers. Two types of labelling chamber set-ups for plants are generally used with 13C stable isotopes. The first type is commercially available, automatic cultivation chambers with a built-in regulation module for environmental parameters such as light, temperature and humidity. The second type, as offered by Vienna Scientific, makes use of custom-made labelling chambers placed in growth chambers or glasshouses which external light, temperature, and gas concentration regulation. Both approaches equally enable continuous monitoring and regulation of atmospheric parameters during labelling. Commonly conducted enrichment of other isotopes such as 15N or 34S does not require closed systems, but systems allowing to add labelled liquids. Generally, both equipment types are suitable to perform pulse labelling and uniformly, long-term labelling of plants towards different isotope enrichment.
See above concerning further general recommendation for respiration chamber designs. Contact us to discuss possible isotope labeling chamber designs that meet your scientific objectives.
|Isotope Labeling Chambers