BLT-PlantView-230F Chlorophyll Fluorescence Imager. The in vivo imaging system for modulated chlorophyll fluorescence employs an ultra-high-speed digital camera featuring an imaging area of 150 mm × 94 mm, offering both high temporal and spatial resolution. This seamlessly integrated system enables micro-measurements of algae cells and fluorescence imaging analyses of seeds, fruits, leaves or entire plants, such as Arabidopsis. Utilizing sophisticated software algorithms, the system proficiently gauges multiple fluorescence parameters, providing insights into the dynamic processes and changes in photosynthesis and the physiological state of plant samples through fluorescence measurements. The measurement process is swift and uncomplicated, ensuring minimal disruption to the samples. Application range includes screening of mutants and varieties for breeding, effects of abiotic stress and pathogens on leaf viability / leaf physiology, effects of storage conditions on fruit quality parameters, etc. Photosynthetic parameters can be assessed via Fv/Fm, ΦPSII, ETR etc. The system can also be easily adapted for green fluorescent protein (GFP) imaging. In sum, the versatile fluorescence imaging system covers a very wide range of applications from single cells, to agriculture, to plant ecology and stress physiology.
Chlorophyll fluorescence is a phenomenon that occurs when chlorophyll molecules absorb light energy during photosynthesis and re-emit some of this energy as fluorescent light. The process involves the excitation of the chlorophyll molecules by the absorbed photons, resulting in the excitation of electrons to higher energy states within the chlorophyll molecule. When these excited electrons return to their ground state, excess energy is released in the form of fluorescence. Main steps in the mechanism of chlorophyll fluorescence include:
The efficiency of this process, known as quantum yield, provides information about the physiological state of the plant and its ability to use the absorbed light energy for photosynthesis.
Chlorophyll fluorescence serves as a highly valuable tool in various scientific and applied plant sciences, including agricultural, forestry and environmental monitoring applications, providing insight into the photosynthetic efficiency and overall health of plants. Some key applications include: