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Dissolved Oxygen O2 Sensors

We offer both optical and polarographic dissolved oxygen sensors manufactured by Qubit Systems. The complete and competitively priced dissolved oxygen Q-OX1LP package, and the individual polarographic dissolved O2 electrodes are particularly useful for studying respiration in small submerged organs (e.g. roots), isolated cells, or microorganisms. For larger aquatic organisms that use intermittent flow respiration, e.g. the Q-Box AQUA aquatic respiration package uses an optical dissolved oxygen probe.

Q-OX1LP Dissolved Oxygen Measurement and Experimentation System

Q-OX1LP Dissolved Oxygen Package
A255 Magnetic Stirrer with O2 electrode cuvette chamber

Q-OX1LP Dissolved Oxygen Package, using a polarographic dissolved oxygen electrode and is designed for measuring photosynthesis and/or respiration in the aqueous phase. The instrument can be employed with suspensions of plant cells, animals, organelles (such as chloroplasts and mitochondria), submerged roots, and algae. Additionally, the Q-OX1LP is well-suited for monitoring any chemical and biochemical reactions that involve the production or consumption of oxygen in the aqueous phase. The Dissolved O2 Package is inclusive of all necessary instruments, accessories, software, data acquisition interface, and manuals, catering to both researchers and students alike.

Continue reading on the OX1LP

The OX1LP Dissolved Oxygen Packages offer a range of DO electrodes with varying volumes, starting from 1 ml up to 50 ml. The O2 electrode, housed in a water-jacketed cuvette, is suitable for measuring O2 consumption (or production) in biological samples or enzymatic reactions. Calibration is a straightforward 2-point process, with all necessary calibration accessories included in the package. With an outstanding accuracy and a resolution of 0.03% O2 (based on air-saturated water = 100%; Clark type), the Dissolved Oxygen Package allows for flexible expression of Dissolved O2 values in desired units such as mg L-1, % dissolved O2, ppm, etc., through a simple calibration procedure. The electrodes have a fast response time (15 s). The water jacket facilitates calibrations and experiments at consistent temperatures; for measurements at different temperatures a convenient correction can be applied using the included software.

Q-OX1LP O2 Analysis Package - Features

Dissolved Oxygen Electrode (Clark Type), with jacket sleeve for temperature control
Dissolved Oxygen Electrode (Clark Type), schematics
  • Polarographic Oxygen Electrode System
  • Outstanding accuracy and resolution 
  • Different volume cuvette available (1, 2.5, 4, 6, 30, 50 ml)
  • rapid response time (15 s)
  • Water jacketed cuvette allows temperature control with optional, circular laboratory water bath
  • All accessories and software included
  • Easy to fit and calibrate

Components Q-OX1LP Dissolved Oxygen Analyzer System OPEN

Table 1: Components of the Q-OX1LP Dissolved Oxygen Analyser System. Please note that the System is available with differently sized cuvettes - the max. volume of the electrode is indicated while the minimum volume is 1/6 of the maximum.

 

Product No. Description
Q-OX1LP-1 Package incl. all components (below) and a dissolved Oxygen Cuvette Electrode cuvette size of 1 ml, perspex
Q-OX1LP-2.5 DO cuvette size: 2.5 ml (glass or perspex)
Q-OX1LP-4 DO cuvette size: 4 ml (perspex)
Q-OX1LP-6 DO cuvette size: 6 ml (glass)
Q-OX1LP-30 DO cuvette size: 30 ml (perspex)
Q-OX1LP-50 DO cuvette size: 50 ml (glass or perspex)
  The different systems above include the cuvette and electrode and the following items: 
Q-A231 Cuvette Electrode Amplifier
Q-A255 Magnetic Stirrer
 Q-OX1LP-SO Stir Bar (2) and O-Ring (2)
 Q-OX1LP-KCl KCl Filling Solution
 Q-OX1LP-MM Membrane Material and Filter Paper
 Q-OX1LP-Tube Excelon Tubing for the Water Jacket
 Q-C610 LabQuest Mini Data Interface
 Q-C901 Logger Pro Software
 Q-OX1LP-CSS Customized Setup Software
 Q-OX1LP-Manual Instructor’s and Student’s Manuals (including experiments)
   
Cuvettes For replacement or additional size
Q-G108 1 ml, perspex
Q-G105 / Q-G105-GL 2.5 ml, perspex / 2.5 ml, glass
Q-G110 4 ml, perspex 
Q-106-GL 6 ml, glass
Q-G109 30 ml, perspex;
Q-G107 / Q-G107-GL 50 ml, perspex / 50 ml, glass
Q-G90-FL 100 µl, flow through
   
Optional items  
Q-A141 Syringes for addition of metabolites to cuvette; 10 µL
Q-A143 Syringes 50 µL
Q-A145 Syringes 100 µL
Q-OX1LP-Therm Thermistor for measurements of sample temperature (not available for 1 ml cuvette)
Q-A133 Calibrated LED light source (Measurement of algae, submerged plants photosynthesis, etc.)
Q-OX1LP-Case Rugged case for storage and transport of all components
   
References Dissolved Oxygen Electrodes OPEN

  • Bessemer et al. (2014) Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology, 1743-5404
  • Koblizek M et al (2020) Utilization of Light energy in phototropic Gemmatimonadetes. Journal of Photochemistyr and Photobiology B 213: 112085 https://doi.org/10.1016/j.jphotobiol.2020.112085
  • Nair P, Huertas M, Nowlin WH (2020) Metabolic responses to long-term food deprivation in subterranean and surface amphipods. Subterranean Biology 33: 1-15 https://digital.library.txstate.edu/handle/10877/9805
  • Henry EF, MacCormack TJ (2018) Taurine protects cardiac contractility in killifish, Fundulus heteroclitus, by enhancing sarcoplasmic reticular Ca2+ cycling. Journal of Comparative Physiology B 188:89-99 https://link.springer.com/article/10.1007/s00360-017-1107-4
  • Lamarre SG et al. (2019) Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
  • Sirikhachornkit et al. (2016) Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient Mutants. J. Microbiol. Biotechnol. 26(5), 854–866
  • Croston, Tara L., et al. (2014) Functional deficiencies of subsarcolemmal mitochondria in the type 2 diabetic human heart. American Journal of Physiology-Heart and Circulatory Physiology 307.1 H54-H65
  • Sussarellu, Rossana, et al. (2013) Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569
  • Dickinson GH et al. (2013) Environmental salinity modulates the effects of elevated CO2 levels on juvenile hard-shell clams, Mercenaria mercenaria. The Journal of experimental biology 216.14 2607-2618.
  • Bauer I and Kappler A (2009) Rates and Extent of Reduction of Fe(III) Compounds and O2 by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908
  • Mosher CM et al. (2008) Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry 47, 45, p11725–11734
  • Mendelsohn BA et al. (2008).The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, 7, p1780–1788 
  • Mendelsohn BA and Gitlin JD (2008) Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, 7, p1789–1798.
  • Johnson EA, Rosenberg J, McCarty RE. (2007) Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380
  • Johnson E A. (2008) Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas reinhardtii. Photosynth Res vol 96:153-162
  • Locuson CW, Gannett PM and Tracy TS (2006) Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, 1-2, p115-129;
Q-OX1LP with DO and Stirrer
Storage case Q-OX1LP
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Dissolved Oxygen Cuvette Electrode (Clark-type)

Dissolved oxygen (DO) cuvette electrode

Dissolved oxygen (DO) cuvette electrodes, DO systems use a polarographic sensing system to measure the production or consumption of dissolved oxygen in liquid suspension. The DO cuvette electrode consists of a water-jacketed cuvette, a removable base containing the electrode and a plunger. These electrodes are available in different volumes: 1, 2.5, 4, 6, 30 and 50 ml, with a 100 µl volume flow-through cuvette also available. The DOCE use a Clark cell type dissolved oxygen detector capable of detecting changes as low as 0.03% O2 in water saturated with 100% air, holding a rapid response time of 15 seconds. Electrodes are used in conjunction with the Q-A231 Electrode Amplifier, Q-A255 Magnetic Stirrer, Q-C610 Data Interface and Q-C901 Software. They are essential components of the Q-OX1LP Dissolved Oxygen package (see above). 

Types/Models Dissolved Oxygen Cuvette Sizes / Materials OPEN

The following DO cuvette electrodes are available, the maximum volume of the electrode is indicated. The minimum volume is 1/6 of the maximum and can be achieved by lowering the plunger into the sample chamber.

 

Product No. Description
Q-G108 DO cuvette size: 1 ml (perspex)
Q-G105-GL / Q-G105 DO cuvette size: 2.5 ml (glass, or perspex)
Q-G110 DO cuvette size: 4 ml (perspex)
Q-G106-GL DO cuvette size: 6 ml (glass)
Q-G109 DO cuvette size: 30 ml (perspex)
Q-G107 DO cuvette size: 50 ml (glass or perspex)
G90-FL DO cuvette size: 100 µl flow through
   
Q-A231 Cuvette Electrode Amplifier
Q-A255 Magnetic Stirrer
Q-C610 LabQuest Mini Data Interface
Q-C901 Logger Pro Software

Q-S122 Optical Measurement of Dissolved O2

Q-S122 Optical Dissolved Oxygen Probe
Q-S122

Q-S122 Optical Dissolved Oxygen Probe. Using luminescence technology, Qubit's optical dissolved oxygen (DO) probe provides fast, easy and accurate measurements of dissolved oxygen in solutions. The versatile Q-S122 probe is well suited for both laboratory and field measurements of dissolved O2 and offers advantages such as no warm-up time, infrequent calibration, no solution to fill, and no stirring. The Q-S122 optical DO probe is a key component of the Q-AQUA aquatic respirometry package and the Qubit DO water control system. With built-in temperature and pressure compensation, it can record DO in % saturation or mg L-1. Submersible up to 5 feet (~1.5 m) with an optional guard for protection and weighting, this probe is recommended for use with the Q-C610 LabQuest Mini data interface and Q-C901 LoggerPro software, or independently with the LabQuest3 data acquisition system. Applications of the Q-S122 DO probe include aquatic respiration, environmental monitoring incl. aquaculture, and water control tasks.

Optical O2 Sensor Q-S122 - Features

  • Luminescence technology
  • Easy to use
  • No warm up required
  • Infrequent calibration
  • No filling solutions required
  • No stirring required
Specifications Optical O2 Probe Q-S122 OPEN

Range: 0 to 20 mg L-1 (or ppm), 0 to 100%
Accuracy: ±0.1 mg L-1 below 10 mg L, ±0.2 mg L-1 above 10 mg L-1, ±1% of reading
Response Time: 90% of final reading in 40 second
12-bit Resolution: 0.006 mg L-1
Temperature Compensation: automatic from 0-50°C
Pressure Compensation: automatic from 30.4 kPa (228 mm Hg) to 202.5 kPa (1519 mm Hg)
Minimum sample flow: none

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Overview - Methods to measure dissolved Oxygen

Several methods have been developed to measure dissolved oxygen in water / liquid samples, each with its own advantages and limitations. Among the key methodologies are: Continue reading
  • Winkler method: The Winkler method is a classic titration-based technique. It involves the addition of reagents to a water sample to convert dissolved oxygen to a measurable form. Titration determines the amount of reagent consumed, indirectly indicating the concentration of dissolved oxygen.
  • Membrane electrode method: This method uses a dissolved oxygen probe equipped with a semi-permeable membrane. The membrane selectively allows oxygen to diffuse through, creating a potential difference. This potential is then measured, providing a direct reading of the dissolved oxygen concentration.
  • Chemiluminescence: Chemiluminescent methods involve the reaction of dissolved oxygen with a specific reagent, resulting in the emission of light. The intensity of the light emitted is proportional to the concentration of dissolved oxygen, allowing accurate measurements.
  • Optical sensors: Optical sensors work on the principle of fluorescence or luminescence. A sensor shines light into the water sample and the amount of light absorbed or emitted by oxygen-sensitive compounds is measured. This provides a rapid and continuous assessment of dissolved oxygen levels.
  • Amperometric sensors: Amperometric sensors measure the current generated when oxygen reacts with an electrode. The current is proportional to the oxygen concentration in the sample, providing real-time monitoring capabilities.
  • Mass spectrometry: Mass spectrometry involves the ionisation and analysis of oxygen molecules. Although it provides highly accurate results, it is typically used in research settings due to its complexity and cost.

The choice of method depends on factors such as the required accuracy, sample size and the presence of interfering substances. See below for selected references on oxygen measurement techniques.

References Methods Dissolved Oxygen Measurements OPEN

  • Clark, L.C., & Lyons, C. (1962). "Electrode systems for continuous monitoring in cardiovascular surgery." Annals of the New York Academy of Sciences, 102(1), 29-45.
  • Davies, M. O., Clark, M., Yeager, E., & Hovorka, F. (1959). The oxygen electrode: I. Isotopic investigation of electrode mechanisms. Journal of The Electrochemical Society, 106(1), 56.
  • Fatt, I. (1964). An ultramicro oxygen electrode. Journal of Applied Physiology, 19(2), 326-329.
  • Nakamura, H., Abe, Y., Koizumi, R., Suzuki, K., Mogi, Y., Hirayama, T., & Karube, I. (2007). A chemiluminescence biochemical oxygen demand measuring method. Analytica chimica acta, 602(1), 94-100.
  • Park, J., Chang, J. H., Choi, M., Pak, J. J., Lee, D. Y., & Pak, Y. K. (2007, October). Microfabirated clark-type sensor for measuing dissolved oxygen. In SENSORS, 2007 IEEE (pp. 1412-1415). IEEE.
  • Ruzgas, T., & Gorton, L. (2000). "A decade of mediator sensors for glucose, lactate, and urea determination." Journal of Electroanalytical Chemistry, 484(2), 179-190.
  • Stauffer, T. B., & Barber, L. B. (2008). "Dissolved oxygen isotope ratio detection of low-level oxygen-18 enriched water." Rapid Communications in Mass Spectrometry, 22(24), 4047-4053.
  • Stetter, J. R., & Li, J. (2008). Amperometric gas sensors a review. Chemical Reviews, 108(2), 352-366.
  • Suzuki, H., Sugama, A., & Kojima, N. (1990). Miniature Clark-type oxygen electrode with a three-electrode configuration. Sensors and Actuators B: Chemical, 2(4), 297-303.
  • Uchida, T., & Suzuki, T. (2010). "Principles of optical chemical sensors for water quality monitoring." Analytical Sciences, 26(12), 1219-1228.
  • Winkler, L. (1888). "Die Bestimmung des im Wasser gelösten Sauerstoffs." Berichte der deutschen chemischen Gesellschaft, 21(3), 2843-2854.
  • Wise, R. R., & Naylor, A. W. (1985). Calibration and use of a Clark-type oxygen electrode from 5 to 45 C. Analytical biochemistry, 146(1), 260-264.
  • Wu, C. C., Luk, H. N., Lin, Y. T. T., & Yuan, C. Y. (2010). A Clark-type oxygen chip for in situ estimation of the respiratory activity of adhering cells. Talanta, 81(1-2), 228-234.

Dissolved CO2 (DCO2) System

For measurements of dissolved CO2 in liquids / suspensions, the
Q-DCO2 System
is available in 2+ configurations (standard/rapid).


Aquatic Animal Respirometry

The Q-AQUA Aquatic Respirometry Package, using the DO probe described above, is designed for measurements inside aquatic respirometry chambers.


Water Properties Control System Research

The Q-ACS Aquatic Control System allows the independent monitoring and control of dissolved oxygen in up to 4 water tanks.


Oxygen Analyser Model

If you are interested in O2 in the gas phase and not in liquid media, consider the gas-phase O2 sensors.



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