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Automated Liquid feeding system for Insect larvae Rearing system

While tray and rack systems are able to successfully and efficiently rear hundred thousands mosquitoes per rack, manual daily application of the liquid feeding solution to larvae requires considerable time. To make the rearing process cost-efficient, and to standardize liquid feed application, Vienna Scientific developed the IR-401 / IR-402 automated tray feeding stations for the established FAO/IAEA-style mosquito tray and rack systems.

Mosquito Tray Feeder for Mass Rearing

Larval rearing and feeding station for mosquitos, automated insect feeder
IR-401 Tray & rack feeding station (liquids) for insect larvae

IR-401 / IR-402 Automatic tray feeding station, designed to deliver precise and consistent amounts of liquid feed to larvae rearing trays, reducing the labour required for feeding and minimising the risk of human error or contamination. The larvae feeders consist of three subsystems: i) Regulated up and down motion for precise fit of the nozzle system that injects the feed mixture, including an automated gauging system to adjust the position between trays, ii) fully automatic feed injection system, driven by microstepping motors, with automatic infeed and outfeed system and uniform feed application on both sides of the trays; and iii) Agitator (to prevent sedimentation) and Pump System for the feed mixture - with a volume of 50 litres. The feeding station can be easily moved (on 4 wheels) and positioned in front of another rack to continue the feeding process. The feeding station is available in two standard configuration for 30 trays, IR-MMR-TR30 (IR-401) and XL racks with 50 trays (IR-402); contact us to discuss customisation. The feed reservoir (50 L, stirred) can be refilled while the system is operating, and both the reservoir and tubing systems can be easily cleaned / rinsed with water after use. The amount of feed can be adjusted. In summary, the IR-401 / IR-402 feeder allows precise and consistent amounts of feed to be delivered to the mosquito larvae, significantly reducing the labour required for feeding and minimising the risk of human error and tray contamination (as the feeder nozzles do not touch the trays).

Mosquito Feeding Station IR-401 / IR-402 - Features

  • Cost effective mosquito tray feeding - once started, the operation runs unattended
  • Easy to use and clean
  • Optimal feed homogenisation and standardised feed quantity (adjustable), large feed container (50 L)
  • Mobile, one feeder can serve dozens of racks / modules

Options:

  • Model for XL tray & rack system with 50 trays (IR-402)
  • Adaptation of the system to an existing insect rack system, customer specific
Models/Types Mosquito Feeding Station OPEN

Product number Description
IR-401 Automatic tray feeding unit for IR-MMR-TR30 racks, liquid feeding systems for 30 trays per rack, 50 l feed container, stirred (stainless), on 4 wheels (turnable)
IR-402 Automatic tray feeding unit XL for IR-MMR-TR50 racks, liquid feeding systems for 50 trays per rack,  50 l feed container, stirred (stainless), on 4 wheels (turnable)
Customization Insect Feeding Station OPEN

With insect-based feeds already approved for pet food and aquaculture, becoming an effective and environmentally friendly on-site protein source for the animal feeds of the future, and a growing interest in supplementing human diets, the application of VSI's innovative liquid feeding technology to other insect rearing tasks is feasible with reasonable effort. Automatic feeding systems for solid feet dispension to insect production racks are under development. Contact us to discuss custom-made solutions for automated liquid or solid insect feed application.

Reference Automatic Mosquito Feeding Station OPEN

The versatility of the IR-401 / IR-402 system in mass rearing facilities has been tested and acknowledged by the IAEA, e.g. "improvements in the equipment necessary for easy handling (automation of the larval rack and addition of a feeding station) have greatly reduced the time, labor, space, and costs associated with rearing large numbers of mosquitoes" (Mamai et al., 2018).

 

  • Mamai, W., et al. 2018. Optimization of mass-rearing methods for Anopheles arabiensis larval stages: effects of rearing water temperature and larval density on mosquito life-history traits. J Econ Entomol 111: 2383-2390.

 

Automated Insect Larvae Tray and Rack Feeding Station IR-401
Mosquito Feeding Station, side view
Mosquito Feeding Station, back view
Mosquito Feeding Station, operation panel
Insect Feeding station, detail feed tank (strirred)
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Importance of the Feeding Method in rearing Mosquito Larvae

Inadequate feeding of mosquito larvae leads to several negative consequences: (1) their development is delayed, (2) their ecdyses becomes irregular, (3) their mortality rate increases, and (4) substandard adults are yielded. On the other hand, providing an excess of food can result in the formation of a layer of scum on the surface of the water-filled rearing trays. Preventing the formation of scum, an abundant food supply would greatly enhance larval growth. The following suggestions for mosquito larvae feeding are generally proposed: ... Continue reading

  1. Use of a wide rearing tray that provides a large surface area.
  2. Provide feed intermittently, distributing it frequently throughout the larval development period and homogeniously on trays, 
  3. Adapt the amount to each developmental stage of larvae, and 
  4. Offer feed in a suspended form.

At the IPCL in Seibersdorf, Austria, for example, Aedes larvae are supplied with a synthetic liquid diet (4% w/v; Puggioli et al. 2013, FAO/IAEA, 2017). This diet was developed to provide all essential nutrients required for optimal larval growth, including fatty acids, proteins, and vitamins. It is composed of a blend of powdered tuna meal (50%), bovine liver (36%), and yeast (14%). Due to variations in the availability and cost of diet components in different countries, locally available and inexpensive diets are however often used. For example, several research and release programs in Brazil, Mexico (Aedes aegypti; Bond et al., 2017), and Italy (Aedes albopictus; Puggioli et al., 2013) have used diet mixtures incorporating Friskies dry adult cat food (Nestle S.A., Switzerland) or rodent diets (PMI Nutrition International LCC, USA). The IAEA strongly recommends that the production of any diets follow standardized procedures to ensure that there is no contamination. In addition, a comprehensive assessment should be conducted to thoroughly evaluate the impact of each diet mixture on mosquito life history traits prior to its use in mass rearing.

To promote synchronized development, larvae diet is is administered at the IAEA laboratories daily from the start of rearing (IAEA/FAO 2020). For example, when using the IAEA large larval tray with 18,000 Aedes larvae, the amount of food provided per tray varies each day for the first nine days. The daily amounts at the IAEA are as follows: 50 mL, 100 mL, 200 mL, 200 mL, 150 mL, 50 mL, 50 mL, 50 mL, and 50 mL, corresponding to 0.11 mg, 0.22 mg, 0.44 mg, 0.44 mg, 0.33 mg, and 0.11 mg of ingredients per larva per day, respectively (Maiga et al., 2019). However, it is possible that different feeding regimens may be tested to ensure optimal quality and to achieve maximum pupation rates during the first few days. To prevent sedimentation of the liquid diet, it is essential to stirr the liquid diet vigorously before each application - which should be evenly distributed on both sides of each large rearing tray.

References Mosquito Mass Rearing / Dietary Requirements OPEN

  • Balestrino, F., M. Q. Benedict, and J. R. L. Gilles. (2012). A New Larval Tray and Rack System for Improved Mosquito Mass Rearing. Journal of Medical Entomology 49:595-605.
  • Balestrino, F., A. Puggioli, J. R. L. Gilles, and R. Bellini. (2014). Validation of a New Larval Rearing Unit for Aedes albopictus (Diptera: Culicidae) Mass Rearing. PLoS ONE 9:e91914.
  • Benedict, M. Q., B. G. Knols, H. C. Bossin, P. I. Howell, E. Mialhe, C. Caceres, and A. S. Robinson. (2009). Colonisation and mass rearing: learning from others. Malaria journal 8:1.
  • Bond, J. G., Ramírez-Osorio, A., Marina, C. F., Fernández-Salas, I., Liedo, P., Dor, A., & Williams, T. (2017). Efficiency of two larval diets for mass-rearing of the mosquito Aedes aegypti. PLoS One, 12(11), e0187420.
  • FAO/IAEA. (2017). Guidelines for routine colony maintenance of Aedes mosquito species. Version.1.0. Seibersdorf, Austria
  • FAO/IAEA. (2020). Guidelines for Mass-Rearing of Aedes Mosquitoes, Hamidou Maiga, Wadaka Mamai, Hanano Yamada, Rafael Argilés Herrero and Jeremy Bouyer (eds.). Vienna, Austria. 24 pp.
  • Feldmann, A., W. Brouwer, J. Meeussen, and J. O. Voshaar. (1989). Rearing of larvae of Anopheles stephensi, using water replacement, purification and automated feeding. Entomologia Experimentalis et Applicata 52:57-68.
  • Kavran, M., Puggioli, A., Šiljegović, S., Čanadžić, D., Laćarac, N., Rakita, M., ... and Bellini, R. (2022). Optimization of Aedes albopictus (Diptera: Culicidae) Mass Rearing through Cost-Effective Larval Feeding. Insects, 13(6), 504.
  • Maïga, H., Mamai, W., Bimbilé Somda, N. S., Konczal, A., Wallner, T., Herranz, G. S., ... & Bouyer, J. (2019). Reducing the cost and assessing the performance of a novel adult mass-rearing cage for the dengue, chikungunya, yellow fever and Zika vector, Aedes aegypti (Linnaeus). PLoS Neglected Tropical Diseases, 13(9), e0007775.
  • Mamai, W., et al. 2018. Optimization of mass-rearing methods for Anopheles arabiensis larval stages: effects of rearing water temperature and larval density on mosquito life-history traits. Journal of Economic Entomology 111(5): 2383-2390.
  • Puggioli, A., Balestrino, F., Damiens, D., Lees, R. S., Soliban, S. M., Madakacherry, O., ... & Gilles, J. R. L. (2013). Efficiency of three diets for larval development in mass rearing Aedes albopictus (Diptera: Culicidae). Journal of Medical Entomology, 50(4), 819-825.
  • Sasmita, H. I., Tu, W. C., Bong, L. J., & Neoh, K. B. (2019). Effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male Aedes aegypti (Diptera: Culicidae): optimizing rearing techniques for the sterile insect programmes. Parasites & vectors, 12(1), 1-16.
  • Van Schoor, T., Kelly, E. T., Tam, N., & Attardo, G. M. (2020). Impacts of dietary nutritional composition on larval development and adult body composition in the yellow fever mosquito (Aedes aegypti). Insects, 11(8), 535.
  • Wang, Y., Eum, J. H., Harrison, R. E., Valzania, L., Yang, X., Johnson, J. A., ... and Strand, M. R. (2021). Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development. Proceedings of the National Academy of Sciences, 118(15), e2101080118.
  • Williges, E., A. Farajollahi, J. J. Scott, L. J. Mccuiston, W. J. Crans, and R. Gaugler. (2008). Laboratory colonization of Aedes japonicus japonicus. Journal of the American Mosquito Control Association 24:591-593.

Mosquito Tray and Rack Systems, collection process

The IR-401 / IR-402 feeding systems are designed for Mosquito Tray and Rack Mass Rearing Systems, with 30 / 50 Trays stacked per rack.


Insect Farming Racks

For insects that require dry substrates in insect rearing systems (such as mealworms), see our Tray and Rack Farming Systems. We offer to develop customised feeding systems for any type of insect rearing system.



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