Rhizobox Systems

Study the "hidden half" of Plants and the Rhizosphere in VSI Rhizobox Systems


Rhizobox, Root box, root imaging boxes, root growth study. rhizosphere study
Design examples rhizoboxes, A4-size.

VSI Rhizobox / RhizonBoxes / root box systems are used to i) separate rhizosphere soil from bulk soil, ii) are a feasible way to study different aspects of soil - soil pore water  - root interactions, iii) to measure root growth, turnover and depth distribution and iv) to sample defined root types (e.g. age classes, orders) or soil areas (rhizosphere / bulk soil). The integration of RRPs' (Micro-)Rhizons and Rhizon Irrigators into our established Rhizobox systems ("Rhizonboxes") creates advanced opportunities for soil water sampling and manipulation.

 

Roots on rhizo(n)box images can be analysed with free or commercial software programs (see bottom of page), however, rhizo(n)boxes are also an educative way to illustrate both root and shoot growth to younger students ("root viewer").

 

Continue reading on Rhizoboxes & RhizonBoxes / and access the Rhizobox configuration sheet.

Sugar beet cultivar imaged with RGB camera. (c) Image property of Gernot Bodner, BOKU.
Sugar beet cultivar imaged with RGB camera. (c) Image property of Gernot Bodner, BOKU.

Large rhizobox imaging. We are currently in the final phase of developing an imaging system for large rhizoboxes not fitting commercially available scanners (A4, A3).  This is foreseen to solve stitching or reflection problems as common with camera-based imaging (see image on the left) and to increase the user friendliness, repeatability and interpretability (especially in regard to segmentation).  Stay tuned.

Software to analyse Roots in Rhizoboxes (examples)

Please check the great Plant Image Analysis Webpage (carefully collected by Guillaume Lobet and colleagues) for further software packages allowing root analysis.

[Vienna Scientific Instruments is not responsible for the content of external links. ]

Selected studies using rhizoboxes / root boxes for Root Phenotyping, Plant and soil studies

  • Beyer, F., D. Hertel, K. Jung, A.-C. Fender, and C. Leuschner. 2013. Competition effects on fine root survival of Fagus sylvatica and Fraxinus excelsior. Forest Ecology and Management 302:14-22.
  • Bontpart, T., C. Concha, V. Giuffrida, I. Robertson, K. Admkie, T. Degefu, N. Girma, K. Tesfaye, T. Haileselassie, A. Fikre, M. Fetene, S. A. Tsaftaris, and P. Doerner. 2019. Affordable and robust phenotyping framework to analyse root system architecture of soil-grown plants. bioRxiv:573139.
  • Gonkhamdee, S., A. Pierret, J. L. Maeght, V. Serra, K. Pannengpetch, C. Doussan, and L. Pagés. 2010. Effects of corn (Zea mays L.) on the local and overall root development of young rubber tree (Hevea brasiliensis Muel. Arg). Plant and Soil 334:335-351.
  • Hylander, L. D. 2002. Improvements of rhizoboxes used for studies of soil–root interactions. Communications in Soil Science and Plant Analysis 33:155-161.
  • Schmidt, J. E., Lowry, C., Gaudin, A. C. 2018. An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients. J. Vis. Exp. (140), e58674.
  • Spohn, M., A. Carminati, and Y. Kuzyakov. 2013. Soil zymography–a novel in situ method for mapping distribution of enzyme activity in soil. Soil Biology and Biochemistry 58:275-280.
  • Wu, J., et al. 2018. RhizoChamber-Monitor: a robotic platform and software enabling characterization of root growth. Plant Methods 14(1): 44.
  • Yao, Q., H. H. Zhu, J. Z. Chen, and P. Christie. 2005. Influence of an arbuscular mycorrhizal fungus on competition for phosphorus between sweet orange and a leguminous herb. Journal of Plant Nutrition 28:2179-2192.